Isotopic composition and concentration of total nitrogen and nitrate in xylem sap under near steady-state hydroponics.
Identifieur interne : 000302 ( Main/Exploration ); précédent : 000301; suivant : 000303Isotopic composition and concentration of total nitrogen and nitrate in xylem sap under near steady-state hydroponics.
Auteurs : Yi Hu [Canada] ; Robert D. Guy [Canada]Source :
- Plant, cell & environment [ 1365-3040 ] ; 2020.
Abstract
After root uptake, nitrate is effluxed back to the medium, assimilated locally, or translocated to shoots. Rooted black cottonwood (Populus trichocarpa) scions were supplied with a NO3- -based (0.5 mM) nutrient medium of known isotopic composition (δ15 N), and xylem sap was collected by pressure bombing. To establish a sampling protocol, sap was collected from lower and upper stem sections at 0.1-0.2 MPa above the balancing pressure, and after increasing the pressure by a further 0.5 MPa. Xylem sap from upper stem sections was partially diluted at higher pressure. Further analysis was restricted to sap obtained from intact shoots at low pressure. Total-, NO3- -N and, by difference, organic-N concentrations ranged from 6.1-11.0, 1.2-2.4, and 4.6-9.4 mM, while discrimination relative to the nutrient medium was -6.3 to 0.5‰, -23.3 to -11.5‰ and - 1.3 to 4.9‰, respectively. There was diurnal variation in δ15 N of total- and organic-N, but not NO3- . The difference in δ15 N between xylem NO3- and organic-N suggests that discrimination by nitrate reductase is near 25.1 ± 1.6‰. When this value was used in an isotope mass balance model, the predicted xylem sap NO3- -N to total-N ratio closely matched direct measurement.
DOI: 10.1111/pce.13809
PubMed: 32463123
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isotopic composition and concentration of total nitrogen and nitrate in xylem sap under near steady-state hydroponics.</title><author><name sortKey="Hu, Yi" sort="Hu, Yi" uniqKey="Hu Y" first="Yi" last="Hu">Yi Hu</name><affiliation wicri:level="4"><nlm:affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Guy, Robert D" sort="Guy, Robert D" uniqKey="Guy R" first="Robert D" last="Guy">Robert D. Guy</name><affiliation wicri:level="4"><nlm:affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32463123</idno><idno type="pmid">32463123</idno><idno type="doi">10.1111/pce.13809</idno><idno type="wicri:Area/Main/Corpus">000284</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000284</idno><idno type="wicri:Area/Main/Curation">000284</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000284</idno><idno type="wicri:Area/Main/Exploration">000284</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isotopic composition and concentration of total nitrogen and nitrate in xylem sap under near steady-state hydroponics.</title><author><name sortKey="Hu, Yi" sort="Hu, Yi" uniqKey="Hu Y" first="Yi" last="Hu">Yi Hu</name><affiliation wicri:level="4"><nlm:affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Guy, Robert D" sort="Guy, Robert D" uniqKey="Guy R" first="Robert D" last="Guy">Robert D. Guy</name><affiliation wicri:level="4"><nlm:affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author></analytic><series><title level="j">Plant, cell & environment</title><idno type="eISSN">1365-3040</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">After root uptake, nitrate is effluxed back to the medium, assimilated locally, or translocated to shoots. Rooted black cottonwood (Populus trichocarpa) scions were supplied with a NO<sub>3</sub><sup>-</sup> -based (0.5 mM) nutrient medium of known isotopic composition (δ<sup>15</sup> N), and xylem sap was collected by pressure bombing. To establish a sampling protocol, sap was collected from lower and upper stem sections at 0.1-0.2 MPa above the balancing pressure, and after increasing the pressure by a further 0.5 MPa. Xylem sap from upper stem sections was partially diluted at higher pressure. Further analysis was restricted to sap obtained from intact shoots at low pressure. Total-, NO<sub>3</sub><sup>-</sup> -N and, by difference, organic-N concentrations ranged from 6.1-11.0, 1.2-2.4, and 4.6-9.4 mM, while discrimination relative to the nutrient medium was -6.3 to 0.5‰, -23.3 to -11.5‰ and - 1.3 to 4.9‰, respectively. There was diurnal variation in δ<sup>15</sup> N of total- and organic-N, but not NO<sub>3</sub><sup>-</sup> . The difference in δ<sup>15</sup> N between xylem NO<sub>3</sub><sup>-</sup> and organic-N suggests that discrimination by nitrate reductase is near 25.1 ± 1.6‰. When this value was used in an isotope mass balance model, the predicted xylem sap NO<sub>3</sub><sup>-</sup> -N to total-N ratio closely matched direct measurement.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32463123</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3040</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>May</Month><Day>28</Day></PubDate></JournalIssue><Title>Plant, cell & environment</Title><ISOAbbreviation>Plant Cell Environ</ISOAbbreviation></Journal><ArticleTitle>Isotopic composition and concentration of total nitrogen and nitrate in xylem sap under near steady-state hydroponics.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/pce.13809</ELocationID><Abstract><AbstractText>After root uptake, nitrate is effluxed back to the medium, assimilated locally, or translocated to shoots. Rooted black cottonwood (Populus trichocarpa) scions were supplied with a NO<sub>3</sub><sup>-</sup> -based (0.5 mM) nutrient medium of known isotopic composition (δ<sup>15</sup> N), and xylem sap was collected by pressure bombing. To establish a sampling protocol, sap was collected from lower and upper stem sections at 0.1-0.2 MPa above the balancing pressure, and after increasing the pressure by a further 0.5 MPa. Xylem sap from upper stem sections was partially diluted at higher pressure. Further analysis was restricted to sap obtained from intact shoots at low pressure. Total-, NO<sub>3</sub><sup>-</sup> -N and, by difference, organic-N concentrations ranged from 6.1-11.0, 1.2-2.4, and 4.6-9.4 mM, while discrimination relative to the nutrient medium was -6.3 to 0.5‰, -23.3 to -11.5‰ and - 1.3 to 4.9‰, respectively. There was diurnal variation in δ<sup>15</sup> N of total- and organic-N, but not NO<sub>3</sub><sup>-</sup> . The difference in δ<sup>15</sup> N between xylem NO<sub>3</sub><sup>-</sup> and organic-N suggests that discrimination by nitrate reductase is near 25.1 ± 1.6‰. When this value was used in an isotope mass balance model, the predicted xylem sap NO<sub>3</sub><sup>-</sup> -N to total-N ratio closely matched direct measurement.</AbstractText><CopyrightInformation>© 2020 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guy</LastName><ForeName>Robert D</ForeName><Initials>RD</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-2573-8226</Identifier><AffiliationInfo><Affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Forest Sciences Centre, Vancouver, British Columbia, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>China Scholarship Council</Agency><Country></Country></Grant><Grant><Agency>Natural Sciences and Engineering Research Council of Canada</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>05</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Cell Environ</MedlineTA><NlmUniqueID>9309004</NlmUniqueID><ISSNLinking>0140-7791</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">black cottonwood</Keyword><Keyword MajorTopicYN="N">isotope discrimination</Keyword><Keyword MajorTopicYN="N">nitrate reductase</Keyword><Keyword MajorTopicYN="N">nitrogen isotopes</Keyword><Keyword MajorTopicYN="N">nitrogen uptake and assimilation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>05</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32463123</ArticleId><ArticleId IdType="doi">10.1111/pce.13809</ArticleId></ArticleIdList><ReferenceList><Title>REFERENCES</Title><Reference><Citation>Altieri, K. 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